Precise Spatial Phenotyping with ChipCytometry Reveals Rare Cells

Researchers identified an uncommon B cell subset associated with autoimmunity.

ChipCytometry is an integrated image-based platform that allows researchers to quantitatively measure high-plex protein biomarker assays with single-cell resolution on both tissue samples and cell suspensions. The technology extensively phenotypes cell populations smaller than what is needed for traditional flow cytometry and retains the spatial context of tissue samples. Samples are stored in a microfluidic chip that allows for convenient sample biobanking and repeated sample interrogation for up to two years. To identify protein biomarkers associated with inflamed tissue, researchers generated single-cell expression data using ChipCytometry. With this technology, they identified a rare B cell subset within intact tissue sections in situ.

Download this application note from Canopy Biosciences to learn how researchers used ChipCytometry to identify a rare immune cell subset.