CRISPR_990x120-1
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Event Overview
A cause for concern regarding the popular CRISPR-Cas9 technology is the potential occurrence of off-target effects. RNA-guided Cas9 may cleave DNA sequences that are not exact complements of the guide strand when either strand harbors bulges due to insertions or deletions. Undoubtedly, a better understanding of the specificity of the CRISPR-Cas9 system can help develop strategies to minimize off-target cleavage. To take a closer look at the specificity and possible causes of off-target effects in the CRISPR-Cas9 system, The Scientist is bringing together researchers who will summarize their work on off-target effects limiting the applications of Cas9-mediated genome modification and discuss strategies for predicting and preventing such off-target effects. Attendees will have the unique opportunity of asking experts about their experience with off-target effects of the CRISPR-Cas9 system.

Topics to be Covered
  • Whole genome sequencing to assess Cas9 off-target effects in genetically engineered mice
  • Will elimination of off-target effects be good enough?
Tuesday, May 7, 2019
 
2:30 - 4:00 PM Eastern Time


Speaker
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Lauryl Nutter, PhD
Assoc. Director, Model Production and Cryopreservation & Recovery
The Centre for Phenogenomics
The Hospital for Sick Children

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David J. Segal, PhD
Professor, Genome Center, Biochemistry & Molecular Medicine
Pharmacology, & MIND Institute
University of California, Davis


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Off-target Effects in CRISPR/Cas9 Genome Editing: Securing Specificity
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