--- title: Combinatorial Single-Cell CRISPR Screens image: https://viewonline.the-scientist.com/hubfs/downloads/TS/TS_10XGenomics/TS_PPL_10x_CRISPR_Research%20Snapshot/New%20Images/1800x640.jpg --- Research Snapshot ![10x Logo on 393939](https://viewonline.the-scientist.com/hubfs/downloads/TS/TS_10XGenomics/TS_PPL_10x_CRISPR_Research%20Snapshot/New%20Images/10x%20Logo%20on%20393939.jpg "10x Logo on 393939") # Combinatorial Single-Cell CRISPR Screens ## Using a scalable direct guide RNA capture method Scientists use single-cell CRISPR screens to explore mammalian gene function and genetic regulatory networks. However, screening relies on vectors that express polyadenylated index transcripts in conjunction with non-polyadenylated guide RNAs. Using specialized capture sequences and unique RT barcodes within partitioning droplets overcomes this problem by directly capturing guide RNAs. ### Download this research snapshot from 10x Genomics to see how researchers at the University of California, San Francisco streamlined CRISPR screening by directly capturing and sequencing guide RNAs in single cells. ## Download the CRISPR Research Snapshot Now ![473x300 Tablet on FFFFFF](https://viewonline.the-scientist.com/hs-fs/hubfs/downloads/TS/TS_10XGenomics/TS_PPL_10x_CRISPR_Research%20Snapshot/New%20Images/473x300%20Tablet%20on%20FFFFFF.jpg?width=473&height=303&name=473x300%20Tablet%20on%20FFFFFF.jpg "473x300 Tablet on FFFFFF") The Scientist c/o LabX Media Group 1000 N West Street, Suite 1200, Wilmington, Delaware, United States, 19801 Toll Free: 888.788.0328 | Phone: 705.528.6888 Email: [privacy@the-scientist.com](mailto:privacy@the-scientist.com) [Privacy Policy](https://www.the-scientist.com/privacy-policy) © 1986-2022 [The Scientist](http://the-scientist.com/)   [![The Scientist Logo\_white](https://viewonline.the-scientist.com/hs-fs/hubfs/The%20Scientist%20Logo_white.png?width=702&name=The%20Scientist%20Logo_white.png "The Scientist Logo_white")](https://www.the-scientist.com) The Scientist needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our [Privacy Policy.](https://www.the-scientist.com/privacy-policy)