Combinatorial Single-Cell CRISPR Screens

Using a scalable direct guide RNA capture method

Scientists use single-cell CRISPR screens to explore mammalian gene function and genetic regulatory networks. However, screening relies on vectors that express polyadenylated index transcripts in conjunction with non-polyadenylated guide RNAs. Using specialized capture sequences and unique RT barcodes within partitioning droplets overcomes this problem by directly capturing guide RNAs.

Download this research snapshot from 10x Genomics to see how researchers at the University of California, San Francisco streamlined CRISPR screening by directly capturing and sequencing guide RNAs in single cells.