Combinatorial Single-Cell CRISPR Screens
Using a scalable direct guide RNA capture method
Scientists use single-cell CRISPR screens to explore mammalian gene function and genetic regulatory networks. However, screening relies on vectors that express polyadenylated index transcripts in conjunction with non-polyadenylated guide RNAs. Using specialized capture sequences and unique RT barcodes within partitioning droplets overcomes this problem by directly capturing guide RNAs.